Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Jia-Ru Wei, Dongchang Xiao, Lei Tang, Nana Xu, Ruifeng Liu, Yuhui Shen, Zihui Xu, Xuan Sang, Jian Ge, Mengqing Xiang, Sheng Liu

Published: 2023-04-11 DOI: 10.1038/s41596-023-00820-z

Neural cell isolation

Adult macaques

Primary brain cells

Neurosphere cultures

High-throughput analyses

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The low number of neural progenitor cells (NPCs) present in the adult and aged primate brains represents a challenge for generating high-yield and viable invitro cultures of primary brain cells. Here we report a step-by-step approach for the fast and rep
Introduction
Materials
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ACKNOWLEDGMENTS

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Neural cell isolation from adult macaques for high-throughput analyses and neurosphere cultures

Extended

Extended Data Fig. 1 Representative figures and electrophysiological recordings demonstrating the viability of acute brain slices in the adult macaque cortex.

Extended Data Fig. 2 Primary culture of cells and labeling assessments from the hippocampus of adult macaque monkeys.

Extended Data Fig. 3 Preparation of fire-polishing glass Pasteur pipettes.

Supplementary information

Reporting Summary

Supplementary Video 1

Supplementary Video 2

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